Drug Metabolism and Pharmacokinetics (DMPK)

Objective

Provide in vitro and in vivo absorption, distribution, metabolism, and excretion (ADME) characterization of small molecule ligands including projected human dosing and drug-drug interaction liabilities 

 

Technology/Techniques 

  • Sciex Triple quadrupoles (4500 with Agilent autoinjector & two 4000’s with LEAP autoinjector PAL HTC)  

  • Sciex QTRAP (5500) with LEAP autoinjector PAL HTC 

  • Tecan Freedom EVO and Agilent Bravo robotic liquid handler 

  • In vitro Clearance; plasma and brain homogenate binding assay  

  • Cytochrome P450 inhibition 

  • Bioanalysis of in vivo PK samples (brain and plasma) 

  • Identification of Metabolites (human/rat)  

 

Innovation 

  • DMPK group typically not found in academic institutions that mimics industrial small Biotech 

  • Full in vitro and in vivo ADME profiling to de-risk preclinical candidates and make early “Go/ No-Go” decisions 

  • Understand mechanisms of clearance and pharmacokinetic/pharmacodynamic (PK/PD) relationships for maximal efficacy on target